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KMID : 0362320100350030152
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Journal of Korean Academy of Operative Dentistry
2010 Volume.35 No. 3 p.152 ~ p.163
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GENE EXPRESSION PROFILING IN HUMAN DENTAL PULP CELLS TREATED WITH MINERAL TRIOXIDE AGGREGATE
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Kim Yong-Beom
Shon Won-Jun
Lee Woo-Cheol
Kum Kee-Yeon
Baek Seung-Ho
Bae Kwang-Shik
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Abstract
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This study investigated the changes in gene expression when mineral trioxide aggregate (MTA) was applied in vitro to human dental pulp cells (HDPCs). MTA in a teflon tube (diameter 10 mm, height 2 mm) was applied to HDPCs. Empty tube-applied HDPCs were used as negative control. For microarray analysis, total RNA was extracted at 6, 24, and 72 hrs after MTA application. The results were confirmed selectively by performing reverse transcriptase polymerase chain reaction for genes that showed changes of more than two-fold or less than half. Of the 24,546 genes, 109 genes were up-regulated greater than twofold (e.g., FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, and TUFT1) and 69 genes were down-regulated below 50% (e.g., SMAD6 and DCN). These results suggest that MTA, rather than being a bio-inert material, may have potential to affect the proliferation and differentiation of pulp cells in various ways.
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KEYWORD
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Microarray, Mineral trioxide aggregate, Dental pulp capping, Human dental pulp cell, Differentiation, Proliferation
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